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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
28/07/2022 |
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Actualizado : |
23/03/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CANOZZI, M.E.A.; CLARIGET, J.M.; ROIG, G.; PEREZ, E.; AZNÁREZ, V.; BANCHERO, G.; LA MANNA, A. |
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Afiliación : |
MARÍA EUGENIA ANDRIGHETTO CANOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN MANUEL CLARIGET BRIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARFRIG Group, Ruta 2 km 288, 65000 Río Negro, Uruguay.; EDUARDO FABIAN PEREZ ARRUTTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARFRIG Group, Ruta 2 km 288, 65000 Río Negro, Uruguay.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO LA MANNA ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Shade effect on behaviour, physiology, performance, and carcass weight of heat-stressed feedlot steers in humid subtropical area. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Animal Production Science, 2022, Volume 62, Issue 17, p.1692-1705. Doi: https://doi.org/10.1071/AN22128 |
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DOI : |
10.1071/AN22128 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted: 13 October 2021; Accepted: 3 June 2022; Published online: 12 July 2022. -- Correspondence author: La Manna, A.; Instituto Nacional de Investigacíon Agropecuaria, INIA La Estanzuela, Ruta 50 km 11, Colonia, Uruguay; email:alamanna@inia.org.uy -- FUNDING: This project was funded by Instituto Nacional de Investigación Agropecuaria (INIA; grant number N-19407, 2016), Uruguay, and by MARFRIG Group industry. -- |
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Contenido : |
Abstract:
Context: Environmental conditions during hot weather decrease feed intake and cattle growth.
Aims: This study conducted over three consecutive years, during the summer, evaluated access to shade as a strategy to mitigate heat stress of finishing cattle in Uruguay.
Methods: Each year, 32 Bos taurus and/or Bos taurus crossbred steers (initially weighing 461 ± 28 kg) were blocked by initial liveweight and assigned to one of eight pens, where one of two treatments were applied, namely, no shade access or shade access (mean shaded area 4.5 m2/animal). Finishing diet was offered three times per day and cattle had ad libitum access to water. Twenty-four pens were considered in a randomised complete-block design. Average daily gain (ADG), dry matter intake (DMI) and feed efficiency were measured, and hot carcass weight was collected at slaughter. Ruminal pH and temperature were obtained using ruminal boluses. Feeding, drinking, rumination, inactive behaviours, respiration rate and painting score were observed.
Key results: Mean days on feed were 70.6 ± 5.5. The average THI ranged from 70 to 72. Steers with access to shade had greater DMI (P = 0.003), ADG (P = 0.004), and feed:gain ratio (P = 0.05) than did not shaded steers. Ruminal pH was higher (P < 0.0001) in shaded than in not shaded animals. The slaughter weight of shaded steers was 10 kg greater (P = 0.02) than that of not shaded steers. A trend (P = 0.06) to higher hot carcass weight after dressing was obtained by shaded steers. The average respiration rate and panting score was consistently lower (P < 0.0001) for shaded than for not shaded steers, except in the early morning. Access to shade increased the time spent by animals feeding (P = 0.008) and ruminating (P = 0.001), decreasing inactive time (P < 0.0001).
Conclusions: Shade could improve performance and welfare of feedlot cattle during summertime, even in regions with an average THI from 70 to 72.
Implications: Access to shade allowed fattening cattle to dissipate heat stored during the day at night and to alleviate the effect of heat load during summer in subtropical region. MenosAbstract:
Context: Environmental conditions during hot weather decrease feed intake and cattle growth.
Aims: This study conducted over three consecutive years, during the summer, evaluated access to shade as a strategy to mitigate heat stress of finishing cattle in Uruguay.
Methods: Each year, 32 Bos taurus and/or Bos taurus crossbred steers (initially weighing 461 ± 28 kg) were blocked by initial liveweight and assigned to one of eight pens, where one of two treatments were applied, namely, no shade access or shade access (mean shaded area 4.5 m2/animal). Finishing diet was offered three times per day and cattle had ad libitum access to water. Twenty-four pens were considered in a randomised complete-block design. Average daily gain (ADG), dry matter intake (DMI) and feed efficiency were measured, and hot carcass weight was collected at slaughter. Ruminal pH and temperature were obtained using ruminal boluses. Feeding, drinking, rumination, inactive behaviours, respiration rate and painting score were observed.
Key results: Mean days on feed were 70.6 ± 5.5. The average THI ranged from 70 to 72. Steers with access to shade had greater DMI (P = 0.003), ADG (P = 0.004), and feed:gain ratio (P = 0.05) than did not shaded steers. Ruminal pH was higher (P < 0.0001) in shaded than in not shaded animals. The slaughter weight of shaded steers was 10 kg greater (P = 0.02) than that of not shaded steers. A trend (P = 0.06) to higher hot carcass weight after dressing was obtained b... Presentar Todo |
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Palabras claves : |
ADG; BEEF CATTLE; EFFICIENCY; FEED INTAKE; HEAT STRESS; MANAGEMENT; PERFORMANCE; TEMPERATURE. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 03499naa a2200313 a 4500
001 1063469
005 2023-03-23
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1071/AN22128$2DOI
100 1 $aCANOZZI, M.E.A.
245 $aShade effect on behaviour, physiology, performance, and carcass weight of heat-stressed feedlot steers in humid subtropical area.$h[electronic resource]
260 $c2022
500 $aArticle history: Submitted: 13 October 2021; Accepted: 3 June 2022; Published online: 12 July 2022. -- Correspondence author: La Manna, A.; Instituto Nacional de Investigacíon Agropecuaria, INIA La Estanzuela, Ruta 50 km 11, Colonia, Uruguay; email:alamanna@inia.org.uy -- FUNDING: This project was funded by Instituto Nacional de Investigación Agropecuaria (INIA; grant number N-19407, 2016), Uruguay, and by MARFRIG Group industry. --
520 $aAbstract: Context: Environmental conditions during hot weather decrease feed intake and cattle growth. Aims: This study conducted over three consecutive years, during the summer, evaluated access to shade as a strategy to mitigate heat stress of finishing cattle in Uruguay. Methods: Each year, 32 Bos taurus and/or Bos taurus crossbred steers (initially weighing 461 ± 28 kg) were blocked by initial liveweight and assigned to one of eight pens, where one of two treatments were applied, namely, no shade access or shade access (mean shaded area 4.5 m2/animal). Finishing diet was offered three times per day and cattle had ad libitum access to water. Twenty-four pens were considered in a randomised complete-block design. Average daily gain (ADG), dry matter intake (DMI) and feed efficiency were measured, and hot carcass weight was collected at slaughter. Ruminal pH and temperature were obtained using ruminal boluses. Feeding, drinking, rumination, inactive behaviours, respiration rate and painting score were observed. Key results: Mean days on feed were 70.6 ± 5.5. The average THI ranged from 70 to 72. Steers with access to shade had greater DMI (P = 0.003), ADG (P = 0.004), and feed:gain ratio (P = 0.05) than did not shaded steers. Ruminal pH was higher (P < 0.0001) in shaded than in not shaded animals. The slaughter weight of shaded steers was 10 kg greater (P = 0.02) than that of not shaded steers. A trend (P = 0.06) to higher hot carcass weight after dressing was obtained by shaded steers. The average respiration rate and panting score was consistently lower (P < 0.0001) for shaded than for not shaded steers, except in the early morning. Access to shade increased the time spent by animals feeding (P = 0.008) and ruminating (P = 0.001), decreasing inactive time (P < 0.0001). Conclusions: Shade could improve performance and welfare of feedlot cattle during summertime, even in regions with an average THI from 70 to 72. Implications: Access to shade allowed fattening cattle to dissipate heat stored during the day at night and to alleviate the effect of heat load during summer in subtropical region.
653 $aADG
653 $aBEEF CATTLE
653 $aEFFICIENCY
653 $aFEED INTAKE
653 $aHEAT STRESS
653 $aMANAGEMENT
653 $aPERFORMANCE
653 $aTEMPERATURE
700 1 $aCLARIGET, J.M.
700 1 $aROIG, G.
700 1 $aPEREZ, E.
700 1 $aAZNÁREZ, V.
700 1 $aBANCHERO, G.
700 1 $aLA MANNA, A.
773 $tAnimal Production Science, 2022, Volume 62, Issue 17, p.1692-1705. Doi: https://doi.org/10.1071/AN22128
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
27/08/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MACÍAS-RIOSECO, M.; SILVEIRA, C.S.; FRAGA, M.; CASAUX, M.L.; CABRERA, A.; FRANCIA, M.E.; REBOLLO, C.; MAYA, L.; ZARANTONELLI, L.; SUANES, A.; COLINA, R.; BUSCHIAZZO, A.; GIANNITTI, F.; RIET-CORREA, F. |
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Afiliación : |
MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Posgrado en Salud Animal, Facultad de Veterinaria, Universidad de la Republica, Montevideo, Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Posgrado en Salud Animal, Facultad de Veterinaria, Universidad de la Republica, Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Posgrado en Salud Animal, Facultad de Veterinaria, Universidad de la Republica, Montevideo, Uruguay.; ANDRÉS CABRERA, Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay.; MARÍA E. FRANCIA, Laboratory of Apicomplexan Biology, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay.; CARLOS REBOLLO, Unidad de Biología Molecular, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay.; LETICIA MAYA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte, Universidad de la República, Gral Fructuoso Rivera 1350, Salto, 50000, Uruguay.; LETICIA ZARANTONELLI, Unidad Mixta Pasteur, Instituto Nacional de Investigación Agropecuaria, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay.; ALEJANDRA SUANES, División de Laboratorios Veterinarios, Ministerio de Ganadería, Agricultura y Pesca, Constituyente 1476, Montevideo, 11200, Uruguay.; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte, Universidad de la República, Gral Fructuoso Rivera 1350, Salto, 50000, Uruguay .; ALEJANDRO BUSCHIAZZO, Laboratorio de Microbiología Molecular y Estructural, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo, 11400, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Causes of abortion in dairy cows in Uruguay. [Causas de aborto em bovinos de leite no Uruguai]. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Pesquisa Veterinaria Brasileira, 1 May 2020, Volume 40, Issue 5, Pages 325-332. OPEN ACCESS. DOI: https://doi.org/10.1590/1678-5150-pvb-6550 |
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DOI : |
10.1590/1678-5150-pvb-6550 |
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Idioma : |
Español |
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Notas : |
Article history: Received on December 3, 2019./Accepted for publication on December 26, 2019. This research was financially supported by Agencia Nacional de Investigación e Innovación (ANII), grant FSSA_X_2014_1_105696. The authors thank Yisell Perdomo, Cecilia Monesiglio, Anderson Saravia,Bruno Lopez, and all graduate students from the animal health platform at INIA, Jennifer McKenna from UW, Francisco Uzal, Karen Sverlow and Juliann Beingesser from CAHFS-UC Davis for valuable assistance. We are grateful to Luis Corbellini, for his keen and valuable suggestions preparing the manuscript, Ximena Salaberry from the Division de Laboratorios Veterinarios (DILAVE) of the Uruguayan ?Ministerio de Ganadería, Agricultura y Pesca (MGAP),Santiago Mirazo from UdelaR, and Cecilia Nieves and Camila Hamond from the Institut Pasteur, Montevideo. |
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Contenido : |
Abstract: A case series study was conducted to determine the frequency of causes of abortion in dairy cattle in Uruguay. The sample size of 102 cases was composed of 53 fetuses, 35 fetuses with placentas, and 14 placentas without an associated fetus. All cases underwent gross
and microscopic pathologic examinations as well as microbiological and serological testing. The etiology was determined in 54 (53%) of cases, 51 of which were caused by infectious agents. Within the observed 102 cases, 30 (29%) were caused by Neospora caninum, six (6%) by Coxiella burnetii and two (2%) by Campylobacter fetus subsp. venerealis. Bovine Parainfluenza-3 virus and Salmonella enterica serovar Newport caused one abortion each. Opportunistic bacteria (Escherichia coli, Streptococcus sp., Staphylococcus sp., Mannheimia sp., Trueperella pyogenes, and Providencia stuartii) were associated with 11 abortions. In two cases the fetal death was attributed to dystocia, and in one case the fetus had a congenital mesothelioma. Bovine viral diarrhea virus (BVDV) infection was identified in three fetuses; two of which were co-infected with and had typical lesions of N. caninum. No lesions were observed in the other fetus infected by BVDV. Leptospira interrogans was identified in one fetus
without lesions. Despite the relatively low overall success rate in establishing an etiological diagnosis in cases of abortion in cattle, a systemic workup of bovine abortion is necessary to establish prevention and control strategies. This also facilitates monitoring and surveillance of reproductive diseases in dairy cattle, some of which represent a risk to public health.
Resumo: Uma série de casos foi estudada para determinar a frequência de causas do aborto em bovinos leiteiros no Uruguai. A amostra, de 102 casos, foi composta por 53 fetos, 35 fetos com placentas e 14 placentas sem feto associado. Todos os casos foram submetidos a exames patológicos macroscópicos e microscópicos, além de testes microbiológicos e sorológicos.A etiologia foi determinada em 54 (53%) dos casos, 51 dos quais foram causados por agentes infecciosos. Nos 102 casos observados, 30 (29%) foram causados por Neospora caninum, seis (6%) por Coxiella burnetii e dois (2%) por Campylobacter fetus subsp. venerealis. O vírus da Parainfluenza-3 e Salmonella enterica serovar Newport causaram um aborto cada. Bactérias oportunistas (Escherichia coli, Streptococcus sp., Staphylococcus sp., Mannheimia sp., Trueperella pyogenes
e Providencia stuartii) foram associadas a 11 abortos. Em dois casos, a morte fetal foi atribuída a distocia e, em um caso, o feto apresentava mesotelioma congênito. A infecção pelo vírus da diarreia viral bovina (BVDV) foi identificada em três fetos; dois dos quais foram co-infectados e apresentavam lesões típicas de N. caninum. Não foram observadas lesões no outro feto infectado pelo BVDV. Leptospira interrogans foi
identificada em um feto sem lesões. Apesar da relativamente baixa taxa de sucesso no diagnóstico etiológico nos casos de aborto em bovinos, é necessário o diagnóstico sistemático dos abortos para estabelecer estratégias de prevenção e controle. Isso também facilita o monitoramento e a vigilância de doenças reprodutivas em bovinos leiteiros, algumas das quais representam um risco para a saúde pública. MenosAbstract: A case series study was conducted to determine the frequency of causes of abortion in dairy cattle in Uruguay. The sample size of 102 cases was composed of 53 fetuses, 35 fetuses with placentas, and 14 placentas without an associated fetus. All cases underwent gross
and microscopic pathologic examinations as well as microbiological and serological testing. The etiology was determined in 54 (53%) of cases, 51 of which were caused by infectious agents. Within the observed 102 cases, 30 (29%) were caused by Neospora caninum, six (6%) by Coxiella burnetii and two (2%) by Campylobacter fetus subsp. venerealis. Bovine Parainfluenza-3 virus and Salmonella enterica serovar Newport caused one abortion each. Opportunistic bacteria (Escherichia coli, Streptococcus sp., Staphylococcus sp., Mannheimia sp., Trueperella pyogenes, and Providencia stuartii) were associated with 11 abortions. In two cases the fetal death was attributed to dystocia, and in one case the fetus had a congenital mesothelioma. Bovine viral diarrhea virus (BVDV) infection was identified in three fetuses; two of which were co-infected with and had typical lesions of N. caninum. No lesions were observed in the other fetus infected by BVDV. Leptospira interrogans was identified in one fetus
without lesions. Despite the relatively low overall success rate in establishing an etiological diagnosis in cases of abortion in cattle, a systemic workup of bovine abortion is necessary to establish prevention and contro... Presentar Todo |
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Palabras claves : |
ABORTO BOVINO; BOVINE ABORTION; BOVINOS DE LEITE; CAMPYLOBACTER FETUS; CATTLE; COXIELLA BURNETII; DAIRY CATTLE; NEOSPORA CANINUM; PLATAFORMA DE SALUD ANIMAL; URUGUAI. |
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Thesagro : |
BOVINOS DE LECHE; URUGUAY. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16698/1/Pesq.-Vet.-Bras.-405p.325-332-May-2020.pdf
https://www.scielo.br/pdf/pvb/v40n5/1678-5150-pvb-40-05-325.pdf
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Marc : |
LEADER 05497naa a2200445 a 4500
001 1061283
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1590/1678-5150-pvb-6550$2DOI
100 1 $aMACÍAS-RIOSECO, M.
245 $aCauses of abortion in dairy cows in Uruguay. [Causas de aborto em bovinos de leite no Uruguai].$h[electronic resource]
260 $c2020
500 $aArticle history: Received on December 3, 2019./Accepted for publication on December 26, 2019. This research was financially supported by Agencia Nacional de Investigación e Innovación (ANII), grant FSSA_X_2014_1_105696. The authors thank Yisell Perdomo, Cecilia Monesiglio, Anderson Saravia,Bruno Lopez, and all graduate students from the animal health platform at INIA, Jennifer McKenna from UW, Francisco Uzal, Karen Sverlow and Juliann Beingesser from CAHFS-UC Davis for valuable assistance. We are grateful to Luis Corbellini, for his keen and valuable suggestions preparing the manuscript, Ximena Salaberry from the Division de Laboratorios Veterinarios (DILAVE) of the Uruguayan ?Ministerio de Ganadería, Agricultura y Pesca (MGAP),Santiago Mirazo from UdelaR, and Cecilia Nieves and Camila Hamond from the Institut Pasteur, Montevideo.
520 $aAbstract: A case series study was conducted to determine the frequency of causes of abortion in dairy cattle in Uruguay. The sample size of 102 cases was composed of 53 fetuses, 35 fetuses with placentas, and 14 placentas without an associated fetus. All cases underwent gross and microscopic pathologic examinations as well as microbiological and serological testing. The etiology was determined in 54 (53%) of cases, 51 of which were caused by infectious agents. Within the observed 102 cases, 30 (29%) were caused by Neospora caninum, six (6%) by Coxiella burnetii and two (2%) by Campylobacter fetus subsp. venerealis. Bovine Parainfluenza-3 virus and Salmonella enterica serovar Newport caused one abortion each. Opportunistic bacteria (Escherichia coli, Streptococcus sp., Staphylococcus sp., Mannheimia sp., Trueperella pyogenes, and Providencia stuartii) were associated with 11 abortions. In two cases the fetal death was attributed to dystocia, and in one case the fetus had a congenital mesothelioma. Bovine viral diarrhea virus (BVDV) infection was identified in three fetuses; two of which were co-infected with and had typical lesions of N. caninum. No lesions were observed in the other fetus infected by BVDV. Leptospira interrogans was identified in one fetus without lesions. Despite the relatively low overall success rate in establishing an etiological diagnosis in cases of abortion in cattle, a systemic workup of bovine abortion is necessary to establish prevention and control strategies. This also facilitates monitoring and surveillance of reproductive diseases in dairy cattle, some of which represent a risk to public health. Resumo: Uma série de casos foi estudada para determinar a frequência de causas do aborto em bovinos leiteiros no Uruguai. A amostra, de 102 casos, foi composta por 53 fetos, 35 fetos com placentas e 14 placentas sem feto associado. Todos os casos foram submetidos a exames patológicos macroscópicos e microscópicos, além de testes microbiológicos e sorológicos.A etiologia foi determinada em 54 (53%) dos casos, 51 dos quais foram causados por agentes infecciosos. Nos 102 casos observados, 30 (29%) foram causados por Neospora caninum, seis (6%) por Coxiella burnetii e dois (2%) por Campylobacter fetus subsp. venerealis. O vírus da Parainfluenza-3 e Salmonella enterica serovar Newport causaram um aborto cada. Bactérias oportunistas (Escherichia coli, Streptococcus sp., Staphylococcus sp., Mannheimia sp., Trueperella pyogenes e Providencia stuartii) foram associadas a 11 abortos. Em dois casos, a morte fetal foi atribuída a distocia e, em um caso, o feto apresentava mesotelioma congênito. A infecção pelo vírus da diarreia viral bovina (BVDV) foi identificada em três fetos; dois dos quais foram co-infectados e apresentavam lesões típicas de N. caninum. Não foram observadas lesões no outro feto infectado pelo BVDV. Leptospira interrogans foi identificada em um feto sem lesões. Apesar da relativamente baixa taxa de sucesso no diagnóstico etiológico nos casos de aborto em bovinos, é necessário o diagnóstico sistemático dos abortos para estabelecer estratégias de prevenção e controle. Isso também facilita o monitoramento e a vigilância de doenças reprodutivas em bovinos leiteiros, algumas das quais representam um risco para a saúde pública.
650 $aBOVINOS DE LECHE
650 $aURUGUAY
653 $aABORTO BOVINO
653 $aBOVINE ABORTION
653 $aBOVINOS DE LEITE
653 $aCAMPYLOBACTER FETUS
653 $aCATTLE
653 $aCOXIELLA BURNETII
653 $aDAIRY CATTLE
653 $aNEOSPORA CANINUM
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aURUGUAI
700 1 $aSILVEIRA, C.S.
700 1 $aFRAGA, M.
700 1 $aCASAUX, M.L.
700 1 $aCABRERA, A.
700 1 $aFRANCIA, M.E.
700 1 $aREBOLLO, C.
700 1 $aMAYA, L.
700 1 $aZARANTONELLI, L.
700 1 $aSUANES, A.
700 1 $aCOLINA, R.
700 1 $aBUSCHIAZZO, A.
700 1 $aGIANNITTI, F.
700 1 $aRIET-CORREA, F.
773 $tPesquisa Veterinaria Brasileira, 1 May 2020, Volume 40, Issue 5, Pages 325-332. OPEN ACCESS. DOI: https://doi.org/10.1590/1678-5150-pvb-6550
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